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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: Long non-coding RNA THOR promotes ovarian Cancer cells progression via IL-6/STAT3 pathway

Fig. 4

THOR promotes ovarian CSCs expansion. a RT- PCR analysis of THOR expression in flow cytometry sorted CD133+ OC cells relative to CD133 OC cells. b OC cell–derived spheroids were trypsinized and cultured in attachment conditions. THOR level in spheroids versus reattached cells was compared by RT- PCR assay. c Representative image of spheres formed from shTHOR and control OC cells. The number of the formed spheres was counted and compared. d The ovarian CSCs markers in shTHOR and control OC cells were determined by real-time PCR. e In vivo limiting dilution assay of shTHOR and control sphere-derived OC cells. (n = 10)

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