Fig. 5

THOR promotes OC cells progression via STAT3 pathway. a The phosphorylation status of STAT3, SMAD3, AKT, and MEK in HO8910 shTHOR or HGSOC shTHOR cells and their control cells was determined by Western blot assays. b The mRNA expression of IL-6, C-myc, Survive and VEGF in HO8910 shTHOR or HGSOC shTHOR cells and their control cells was checked by real-time PCR. c The shTHOR OC cells were infected with THOR overexpression virus, and the overexpression effect was checked by RT-PCR assay. d The shTHOR OC cells were infected with THOR overexpression virus, and then subjected to western blot assay. e The shTHOR OC cells were infected with THOR overexpression virus, and then subjected to RT-PCR assay. f The proliferation of HO8910 shTHOR or HGSOC shTHOR cells and their control cells in the presence of S3I-201 (100 nM) or not was measured using CCK8 assays. g Migration assays were performed using OC shTHOR cells and its control cells in the presence of S3I-201 (100 nM) or not. h Invasion assays were performed using OC shTHOR cells and its control cells in the presence of S3I-201 (100 nM) or not. i Spheroid formation assays were performed using OC shTHOR cells and its control cells in the presence of S3I-201 (100 nM) or not