Fig. 4From: Anti-proliferative activities of Byrsocarpus coccineus Schum. and Thonn. (Connaraceae) using ovarian cancer cell linesEffect of B. coccineus ethanol leaf extract on apoptosis of ovarian cancer cells. OvCa cells were treated with different dosage of B. coccineus extract (OVCAR-3: 446.5 μg/mL; and SW 626: 486.94 μg/mL) for 48 h, and apoptosis was assessed by staining with Annexin V-(conjugated with FITC)/ PI followed by flow cytometry. Quadrant Q1, Q2, Q3 and Q4 represents the percentages of necrotic (Annexin (−)/(PI (+), late (Annexin (+)/(PI (+), early (Annexin (+)/(PI (−) apoptotic, and viable (Annexin (−)/(PI (−) cells, respectively. DMSO was used in untreated cells. Upon treatment with extract, both cell lines showed higher number of apoptotic cells in early apoptotic phase compared to in untreated cellsBack to article page