Fig. 5

Validation of gene expression by RT-PCR for apoptosis, cell cycle and inflammatory markers. OVCAR-3 and SW 626 cells were treated with B. coccineus extract for 24 and 48 h, and mRNA fold change expression of (a) pro-(BID, BAX, BAD), anti- (MCL-1, BCL-2), apoptotic (Caspase-3, PARP), p53 (b) cell cycle regulators (p21, CYCLIND1, CDK2, CDK4), and (c) pro- inflammatory cytokines (TNFα, IL-10) markers, relative to control cells (DMSO used) were analyzed through RT-PCR. 18S, a house keeping gene was used for data normalization. Data presented as the Mean ± SEM, and level of significance was determined by students t- tests. The asterisks * and ** indicates P < 0.05 and P < 0.01, respectively. The experiments were repeated three times