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Fig. 6 | Journal of Ovarian Research

Fig. 6

From: Anti-proliferative activities of Byrsocarpus coccineus Schum. and Thonn. (Connaraceae) using ovarian cancer cell lines

Fig. 6

The expression of pro- and anti-apoptotic markers in ovarian cancer cells through western blots. OVCAR-3 and SW626 cells were treated with B. coccineus extract for 24 and 48 h, and the expression of apoptotic markers were analyzed through the western blots. The immunoblots of pro- (BID, BAD) and anti-apoptotic (MCL-1, BCL-XL) proteins are shown in upper panel, and the densitometry of the these proteins are shown in lower panel. DMSO was used in untreated (UT) cell. The immunoblots showed pro- apoptotic proteins were upregulated and anti-apoptotic were down regulated upon treatment at 48 h compared to 24 h and untreated cells. GAPDH, a house keeping genes was used as loading control to ensure equal loading in each blot. The asterisks ** indicates P < 0.01. The experiments were repeated three times

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