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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cells

Fig. 3

WISP2 deletion affects cell cycle and promotes apoptosis. a WISP2 deletion affected the cell cycle. ES-2 WT and WISP2 deletion cells were cultured overnight. PI (propidium iodine) staining followed by FACS detected the cell cycle stage. b WISP2 deletion promoted apoptosis. ES-2 WT and WISP2 deletion cells were cultured overnight, and 0.5 × 105 cells were stained with PE Annexin V and analyzed via FACS within 1 h. c Loss of WISP2 promoted senescence. ES-2 WT and WISP2 deletion cells were cultured and stained for the senescence marker SA-β-gal. Quantification is shown in the right panel. d WISP2 deletion increased senescence-related gene expression. Expression of p16, Perp, and Mdm2 mRNAs was determined via q-PCR. Data are expressed as the mean ± s.d. from three independent experiments. ** = p < 0.01; one-way ANOVA

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