Fig. 6
From: WISP2 promotes cell proliferation via targeting ERK and YAP in ovarian cancer cells
Loss of WISP2 inhibits ES-2 tumor growth in xenografted mice. a-b Deletion of WISP2 in ES-2 cells inhibited tumor growth in vivo. ES-2 WT and WISP2 deletion cells (1 × 106 cells per each injection) were implanted subcutaneously into nude mice. Tumor volume was monitored, and tumor weight determined at the end of the experiments (n = 8). c Increased expression of YAP/TAZ and ERK1/2 target genes in WISP2 deleted tumors. RNA was isolated from tumor samples (panel B) and analyzed for target genes via RT-PCR. Data normalized relative to the corresponding value of WT tumors are expressed as the mean ± s.d (n = 3 tumors per group). *p < 0.05; **p < 0.01; *** p < 0.001; Student’s t-test. d Loss of WISP2 decreased cell proliferation and increased apoptosis in ES-2 tumors. Immunohistochemical staining for p-ERK1/2, p-YAP, cleaved caspase-3, and p-Histone H3 in representative tumor tissue; Scale bar, 50 μm. e Western blot detected protein expression in WISP2 deleted tumors. Tumor samples (panel B) were harvested and subjected to immunoblot analysis with the indicated antibodies