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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Transcriptomics of cumulus cells – a window into oocyte maturation in humans

Fig. 1

Hierarchical Clustering (HC), Principal Component Analysis (PCA), and Differential Expression (DE) analysis of cumulus cells surrounding mature eggs (MII-CC) compared with cumulus surrounding immature eggs (GV-CC). a A Venn diagram depicting the allocation of total number of genes that were expressed in our study. The overlap represents genes commonly expressed in MII-CC (orange) and GV-CC (blue), and genes unique to one of the cohorts in the periphery. b Samples cluster by corresponding egg maturity under unsupervised HC. The samples are on rows, and the transcripts are on columns, red indicating upregulated expression and green indicating downregulated expression. c PCA of all CC samples shows significant separation along PC1 by oocyte maturity and no apparent effect of patient age, depicted by the size of the sphere. d DE analysis between MII-CC and GV-CC using DESeq2; 1818 genes were differentially expressed (1031 downregulated, in red (FC < -2 and FDR < 0.05), and 787 upregulated, in green (FC > 2 and FDR < 0.05))

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