Fig. 4
From: Transcriptomics of cumulus cells – a window into oocyte maturation in humans
a Validation of RNAseq results by qPCR of 16 targets and normalized to RPLP0 in duplicate. Fold change was calculated using the ∆∆Ct method between the MII-CC and GV-CC cohorts. Results of RNAseq (open bars) and qPCR (filled bars) are presented as fold change between MII-CC and GV-CC samples. b Additional 34 CC from 7 patients (18 MII-CC and 16 GV-CC) analyzed using qPCR for 17 targets (16 genes and one reference gene RPLP0), in duplicate. Normalized ∆Ct values are plotted for each sample, horizontal line represents mean ∆Ct, and error bars represent SEM