Fig. 2

MiR-27b-5p inhibits the proliferation of ovarian cancer cells in vitro. A. SKOV3 and OVCAR3 cells were transfected with miR-NC or miR-27b-5p mimics and the level of miR-27b-5p was analyzed by qRT-PCR assay. B. CCK-8 assay of SKOV3 and OVCAR3 transfected with miR-NC or miR-27b-5p. C. Colony formation assay was conducted to evaluated cell proliferative ability in miR-27b-5p overexpressing SKOV3 and OVCAR3 cells. D. Soft agar colony formation assay was conducted to evaluated cell proliferative ability in miR-27b-5p overexpressing SKOV3 and OVCAR3 cells. ^**P < 0.05 vs. miR-NC. E. SKOV3 cells were transfected with miR-NC inhibitor or miR-27b-5p inhibitor and the level of miR-27b-5p was analyzed by qRT-PCR assay. F. CCK-8 assay of SKOV3 cells transfected with miR-NC inhibitor or miR-27b-5p inhibitor. G. Colony formation assay was carried out using SKOV3 transfected with miR-NC inhibitor or miR-27b-5p inhibitor. ^**P < 0.05 vs. miR-NC inhibitor