Fig. 5

Overexpression of CXCL1 reverses the effects of miR-27-5p. A. SKOV3 and OVCAR3 cells were co-transfected with miR-27b-5p mimics and pc-CXCL1 or pcDNA3.1. The level of CXCL1 was detected by qRT-PCR. B. Western blot analysis was utilized for the detection of CXCL1 protein expression in SKOV3 and OVCAR3 cells following pcDNA3.1 or pc-CXCL1 transfection. C. SKOV3 and OVCAR3 cells were co-transfected with miR-27b-5p mimics and pc-CXCL1 or pcDNA3.1. Following transfection, the proliferations of SKOV3 and OVCAR3 cells were examined using CCK-8 assays D. The colony formation of SKOV3 and OVCAR3 cells were examined. E. The migration of SKOV3 and A2780 cells were examined using wound healing assay. F. The invasion of SKOV3 and OVCAR3 cells were examined using Transwell invasion assay. ^**P < 0.05 vs. miR-NC, ^##P < 0.05 vs. miR-27b-5p + pc-CXCL1