Fig. 1

Pannexin1 expression in Bovine Cumulus-Oocyte Complexes, During Different Follicular Stages, and Varying Stages of Oocyte Developmental Competence. a Representative immunofluorescent images of bovine COCs stained with Hoechst33342 (blue, a) and labeled with PANX1 antibody (green, b). c A representative image depicting a bovine COC stained with Hoechst33342 and stained with secondary antibody only (d). Oocyte outlined with dashed circle. Bars = 100 μm. e Representative blot images depicting the levels of PANX1 expression in granulosa cells isolated from small (< 2 mm) and large (> 5 mm) antral follicles. GAPDH was used as a loading control. f Densitometry results showing the expression level of PANX1 in granulosa cells isolated from small and large antral follicles. g Representative blot images depicting the levels of PANX1 in BCB- and BCB+ COCs. h Densitometry results showing the expression of PANX1 in BCB- and BCB+ groups of COCs. *Denotes a significant difference, p < 0.05. Error bars are ± SD from mean