Fig. 3

ROS and Maturation Effects of Pannexin 1 Inhibition. a Representative images of cumulus expansion after 22 h of IVM in control COCs and PANX1 inhibited COCs. Magnification was 20x. b Cumulus expansion in the control and 10Panx treated groups. c Meiotic maturation proportions of oocytes at the GV, GVBD, and MII stages after 6 h and 22 h of IVM with or without PANX1 inhibition. d The average icAMP concentration of COCs treated with or without 10Panx at 0, 3, 6, and 22 h of IVM. *Denotes a significant difference, p < 0.05. Error bars are ± SD from mean. Representative images depicting the staining intensity of DCFH-DA showing levels of free radicals within the oocyte after 22 h of IVM without (f) or with (h) PANX1 inhibition. Cells were nuclear stained with Hoechst33342 (blue, e & g). Bars = 200 μm. i Quantitation of the staining intensity of DCFH-DA using CTCF. *Denotes a significant difference, p < 0.05. Error bars are ± SD from mean