Fig. 4

Knockdown of RPS6 inhibited ovarian cancer cell migration and invasion in vitro. Migration ability was assessed using the scratch test after 24 and 48 h (magnification 40×; scale bar: 500 μm) on SKOV3 (a, b) and HO-8910 (c, d) cells. Transwell migration assays were conducted to assess the effect of RPS6 knockdown on SKOV3 and HO-8910 cell migration (magnification 100×; scale bar: 200 μm) (e). Graph of transwell migration assay data for SKOV3 cells with RPS6 knocked down (112 ± 4.90 and 122 ± 8.38), showing reduced cell migration, compared with control cells (shCon) (350 ± 12.25) (N = 3, 2-tailed unpaired t-test, p < 0.0001) (f). Graph of transwell migration assay data for HO-8910 cells with RPS6 knocked down (47 ± 5.73 and 63 ± 5.25), showing reduced cell migration, compared with shCon (288 ± 4.64) (N = 3, 2-tailed unpaired t-test, p < 0.0001) (g). Representative images showing Matrigel invasion assays using SKOV3 and HO-8910 cells with RPS6 knocked down and respective controls (magnification, 200×; scale bar: 200 μm) (h). Representative graph of Matrigel invasion assay data for SKOV3 cells with RPS6 knocked down (15.33 ± 0.47 and 24.8 ± 4.95), compared with controls (shCon) (176 ± 20.05) (N = 3, 2-tailed unpaired t-test, p < 0.001) (i). Representative graph of Matrigel invasion assay data for HO-8910 cells with RPS6 knocked down (26.7 ± 3.30 and 19.0 ± 7.93) compared with controls (shCon) (149.1 ± 22.70) (N = 3, 2-tailed unpaired t-test, p < 0.001) (J). Data are presented as mean ± SD for three independent experiments; ***p < 0.001, ****p < 0.0001