Fig. 3

TIAM1 expression was negatively regulated by miR-1271-5p. a-b Relative mRNA expression of TIAM1 was assessed by qRT-PCR in OVCAR-3 and SK-OV-3 cells after different transfections, including inhibitor/mimic, si-TIAM1/TIAM1, inhibitor+si-TIAM1 or mimic+TIAM1, ^**P < 0.01 versus NC group, ^##P < 0.01 versus inhibitor or mimic group, ^&&P < 0.01 versus si-TIAM1 or TIAM1 group. c-d After above transfections, western blot experiment was performed to measure the protein expression level of TIAM1 in OVCAR-3 and SK-OV-3 cells. The gray values of protein bands were quantified. ^**P < 0.01 versus NC group, ^##P < 0.01 versus inhibitor or mimic group, ^&&P < 0.01 versus si-TIAM1 or TIAM1 group