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Fig. 5 | Journal of Ovarian Research

Fig. 5

From: LINC01094/miR-577 axis regulates the progression of ovarian cancer

Fig. 5

Down-regulation of miR-577 expression partially reversed the biological behavior of OC cells induced by knockdown LINC01094. a The expression of miR-577 in SKOV3 and 3AO cells transfected with si-NC, si-LINC01094 or si-LINC01094 + miR-577-in was detected by qRT-PCR assay. b CCK-8 assay was used to detect the proliferation of SKOV3 and 3AO cells transfected with si-NC, si-LINC01094 or si-LINC01094 + miR-577-in. c Transwell assay was used to detect the migration of SKOV3 and 3AO cells transfected with si-NC, si-LINC01094 or si-LINC01094 + miR-577-in. d Transwell assay was used to detect the invasion of SKOV3 and 3AO cells transfected with si-NC, si-LINC01094 or si-LINC01094 + miR-577-in. e Western blot was used to analyze the effects of transfection with si-NC, si-LINC01094, or si-LINC01094 + miR-577-in on the expression of EMT markers. f Western blot was used to detect β-catenin, cyclin-D1 and c-Myc protein expressions in SKOV3 and 3AO cells transfected with si-NC, si-LINC01094 or si-LINC01094 + miR-577-in. All experiments were performed in triplicate. * P < 0.05, ** P < 0.01 and *** P < 0.001

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