Skip to main content
Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Long non-coding RNA PTPRG-AS1 promotes cell tumorigenicity in epithelial ovarian cancer by decoying microRNA-545-3p and consequently enhancing HDAC4 expression

Fig. 1

PTPRG-AS1 silencing inhibits EOC cell proliferation, migration, and invasion but promotes cell apoptosis in vitro. a Expression of PTPRG-AS1 in 56 EOC tissues and 21 normal ovarian surface epithelial tissues was measured by RT-qPCR. b RT-qPCR measurement of PTPRG-AS1 expression in four EOC cell lines (ES-2, OVCAR3, CAOV-3, and SK-OV-3) and human ovarian surface epithelial (OSE) cells. c Overall survival was analyzed in EOC patients according to PTPRG-AS1 expression levels. d The knockdown efficiency of si-PTPRG-AS1 was evaluated by RT-qPCR in OVCAR3 and CAOV-3 cells. e The proliferation of OVCAR3 and CAOV-3 cells after PTPRG-AS1 depletion was detected by CCK-8 assay. f Flow cytometry analysis showed the apoptosis in OVCAR3 and CAOV-3 cells following inhibition of PTPRG-AS1. g, h The migratory and invasive capacities of OVCAR3 and CAOV-3 cells following PTPRG-AS1 knockdown were determined by Transwell cell migration and invasion assays. i The E-cadherin, N-cadherin and Vimentin protein levels in PTPRG-AS1 depleted-OVCAR3 and CAOV-3 cells were examined by western blotting. **P < 0.01

Back to article page