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Fig. 2 | Journal of Ovarian Research

Fig. 2

From: Long non-coding RNA PTPRG-AS1 promotes cell tumorigenicity in epithelial ovarian cancer by decoying microRNA-545-3p and consequently enhancing HDAC4 expression

Fig. 2

PTPRG-AS1“serves as a miR-545-3p” sponge in EOC. a The distribution of PTPRG-AS1 as predicted by lncLocator. b lncATLAS predicts the subcellular localization of PTPRG-AS1. c Subcellular fractionation was performed in OVCAR3 and CAOV-3 cells, after which the cytoplasmic and nuclear fractions were analyzed by RT-qPCR for the determination of PTPRG-AS1 subcellular localization. d The potential miRNAs that interact with PTPRG-AS1 were predicted by miRDB and StarBase 3.0. e The expression of potential target miRNAs in si-PTPRG-AS1-transfected or si-NC-transfected OVCAR3 and CAOV-3 cells was analyzed by RT-qPCR. f miR-545-3p expression in 56 EOC tissues and 21 normal ovarian surface epithelial tissues was measured by RT-qPCR. g Correlation between the expression of miR-545-3p and PTPRG-AS1 in 56 EOC tissues was analyzed by Pearson’s correlation analysis. h The schematic illustration of wild-type and mutant miR-545-3p binding sites within the sequences of PTPRG-AS1. i Luciferase reporter assay in OVCAR3 and CAOV-3 cells after cotransfection with miR-545-3p mimic or NC mimic and PTPRG-AS1-WT or PTPRG-AS1-MUT. j RIP assay was implemented, and relative enrichment of PTPRG-AS1 and miR-545-3p was detected by RT-qPCR. **P < 0.01

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