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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Long non-coding RNA PTPRG-AS1 promotes cell tumorigenicity in epithelial ovarian cancer by decoying microRNA-545-3p and consequently enhancing HDAC4 expression

Fig. 3

HDAC4 is a direct target of miR-545-3p in EOC. a The overexpression efficiency of miR-545-3p mimic in OVCAR3 and CAOV-3 cells was examined by RT-qPCR. b, c CCK-8 assay and flow cytometry analyses were performed to measure the proliferation and apoptosis of OVCAR3 and CAOV-3 cells after miR-545-3p overexpression. d, e Transwell cell migration and invasion assays assessed the migration and invasion of OVCAR3 and CAOV-3 cells that were transfected with miR-545-3p mimic or NC mimic. f The wild-type and mutant putative binding sequences of miR-545-3p in the sequence of the HDAC4 3′-UTR. g, h The expression levels of HDAC4 mRNA and protein in OVCAR3 and CAOV-3 cells with miR-545-3p upregulation were measured by RT-qPCR and western blot analysis. i HDAC4 mRNA expression was measured by RT-qPCR in 56 EOC tissues and 21 normal ovarian surface epithelial tissues. j The relationship between HDAC4 mRNA and miR-545-3p in 56 EOC tissues was determined by Pearson’s correlation analysis. k miR-545-3p mimic or NC mimic was cotransfected with HDAC4-WT or HDAC4-MUT into OVCAR3 and CAOV-3 cells. The luciferase activity was detected at 48 h post-transfection. **P < 0.01

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