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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: Long non-coding RNA PTPRG-AS1 promotes cell tumorigenicity in epithelial ovarian cancer by decoying microRNA-545-3p and consequently enhancing HDAC4 expression

Fig. 4

PTPRG-AS1 sequesters miR-545-3p and thereby increases HDAC4 expression in EOC. a A RIP assay was performed to elucidate whether PTPRG-AS1, miR-545-3p, and HDAC4 exist in the same RISC. b, c RT-qPCR and western blot analysis were conducted to measure HDAC4 mRNA and protein expression in PTPRG-AS1 deficient OVCAR3 and CAOV-3 cells. d, e si-PTPRG-AS1 combined with miR-545-3p inhibitor or NC inhibitor were cotransfected into OVCAR3 and CAOV-3 cells, and the expression changes of HDAC4 mRNA and protein were assessed. f Pearson’s correlation analysis was applied to examine the relationship between PTPRG-AS1 and HDAC4 mRNA expression in 56 EOC tissues. **P < 0.01

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