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Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Comparison of methods for quantifying primordial follicles in the mouse ovary

Fig. 3

Comparison of follicle numbers obtained by direct counts using different fixative and embedding combinations. Follicle numbers in ovaries from mice treated with saline or cyclophosphamide were determined using direct counts. Ovaries were fixed in Bouin’s (a, c) or formalin (b, d) and embedded in paraffin (c, d) or resin (a, b) and sectioned at 5 μm (c, d) or 20 μm (a, b). The analysis shown in D is the same as Fig. 1b, and is included here to enhance interpretation of (a-c). Data are represented as mean ± SEM; standard t-test; n = 5–6/group); ns = non-significant

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