Fig. 4

Comparison of follicle numbers obtained by stereology and direct counts in saline and cyclophosphamide treated ovaries, following different combinations of fixation and embedding material. Follicle numbers in ovaries from mice treated with saline (a-h) or cyclophosphamide (i-p) were compared following stereology or direct counts. Ovaries were fixed in Bouin’s (a-d, i-l) or formalin (e-h, m-p) and embedded in paraffin and sectioned at 5 μm (c, d, g, h, k, l, o, p) or resin and sectioned at 20 μm (a, b, e, f, i, j, m, n). Data are represented as mean ± SEM; standard t-test (A, B, C, G, H, J) or correlation plots carried out using linear regression analysis (d-f, j-l); n = 5–6/group; (d, e, f, i, k, l); n = 5–6/group); ns = non-significant