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Table 2 Studies of ctDNA in ovarian cancer patients related to treatment response monitoring

From: Prediction of the treatment response in ovarian cancer: a ctDNA approach

References year No of patients Identified Abnormalities Methodology
Gifford et al. [121] 2004 138 hMLH1 methylation Microsatellite PCR
Swisher et al. [122] 2005 137 p53 mutation DNA sequencing
Kamat et al. [123] 2006 Level RT-PCR
Capizzi et al. [124] 2008 22 Level RT-PCR
Kamat et al. [125] 2010 164 Beta-globin RT-PCR
Wimberger et al. [126] 2011 62 Fluorimetry Fluorescence
Forshew et al. [127] 2012 38 TP53. Other markers include PTEN, BRAF, KRAS, EGFR, PIK3CA TAm-Seq, dPCR
Murtaza et al. [128] 2013 3 RB1, ZEB2, BUB1, CES4A, MTOR, PARP8 NGS, qPCR
Choudhuri et al. [129] 2014 100 Level RT-PCR
Martignetti et al. [130] 2014 1 FGFR2 fusion transcript RT-PCR
Pereira et al. [131] 2015 22 TP53, PTEN, PIK3CA, MET, KRAS, FBXW7, BRAF WES, ddPCR, TGS
Cohen et al. [132] 2016 32 CNV WES
Harris et al. [133] 2016 10 Aberrant chromosomal junctions RT-PCR
Piskorz et al. [134] 2016 18 TP53 mutation NGS
Parkinson et al. [135] 2016 40 TP53 mutation Digital PCR
Vanderstichele [136] 2017 57 CNV WGS
Phallen et al. [137] 2017 42 55 gene panel including TP53, KIT, ALK, APC, ERBB4 etc NGS (TEC-Seq) and ddPCR
Flanagan et al. [138] 2017 247 Methylation at CpG sites NGS
Widschwendter et al. [139] 2017 151 Regions linked to COL23A1, C2CD4D and WNT6 TUC-BS & RRBS
Ratajska et al. [140] 2017 121 BRCA1/2 mutations NGS
Christie et al. [141] 2017 30 BRCA reversion mutation NGS
Weigelt et al. [116] 2017 19 BRCA reversion mutation NGS
Giannopoulou et al. [142] 2018 50 ESR1 RT-MSP
Du et al. [143] 2018 21 CNV and mutant genes including TP53, BRCA1, NOTCH2, DNMT3A etc NGS
Morikawa et al. [107] 2018 29 KRAS and PIK3CA ddPCR
Nakabayashi et al. [144] 2018 36 CNV WGS
Park et al. [109] 2018 4 TP53 ddPCR
Arend et al. [117] 2018 14 50 gene panel NGS
Lin et al. [145] 2019 97 BRCA reversion mutation, TP53 NGS
Kim et al. [110] 2019 102 TP53 mutant allele Sanger sequencing/Digital PCR
Oikkonen et al. [118] 2019 12 ERBB2 amplification NGS
Iwahashi et al. [146] 2019 4 TP53, APC, BRCA1 and KRAS CAPP-seq
Noguchi et al. [119] 2020 10 gene mutation profiles and blood tumor mutation burden CAPP-seq
Han et al. [147] 2020 10 88 genes panel NGS
Alves et al. [148] 2020 11 Level qPCR
  1. Abbreviations: NGS Next-generation sequencing, RT-PCR Reverse transcription polymerase chain reaction, dPCR droplet Polymerase chain reaction, qPCR Allele-specific quantitative PCR, RT-MSP Real-Time methylation specific PCR, CNV Copy number variation, WGS Whole genome sequencing, WES Whole exome sequencing, ddPCR Droplet digital PCR, TGS Targeted gene sequences, TAm-RSeq Targeted amplicon re-sequencing, RRBS Reduced representation bisulphite sequencing, TUC-BS Targeted ultra-high coverage bisulphite sequencing, CAPP-seq Cancer Personalized Profiling by deep Sequencing