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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Chemoprotective effects of plasma derived from mice of different ages and genders on ovarian failure after cyclophosphamide treatment

Fig. 1

A Schematic representation of the experimental protocol used for the cyclophosphamide (CYC) -induced ovarian failure and treatment by plasma derived from old male plasma (OMP), young female plasma (YFP) and young male plasma (YMP) up to day 19. Histological sections show stereological methods. a Mean value of the 4 different diameters of oocyte, follicle and granulosa cells to estimate diameters, b Optical dissector method by unbiased counting frame on histological section to estimate number, c Cavalieri point-counting method to estimate the volume density of the ovarian structures with fraction of the ovary marked with points over histological section that also specifies the peripheral rim of cortex of the central medulla. B Analysis of animal weight and C ovarian weight in all groups. *and # indicate significant change from CYC and control respectively. Values are Mean ± SD; (* P < 0.05); (**P ≤ 0.001)

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