Skip to main content

Table 2 Effects of endometrioma fluid, transferrin, cysteamine and cystine on mitochondrial metabolism of mouse oocytes

From: Transferrin and antioxidants partly prevented mouse oocyte oxidative damage induced by exposure of cumulus-oocyte complexes to endometrioma fluid

Groups

ATP content (pmol/oocyte)

ADP content (pmol/oocyte)

ADP/ATP

Mitochondrial membrane polarization

1:Control

0.47 ± 0.18 a

0.13 ± 0.05 a

0.28 ± 0.11 a

0.78 ± 0.36 a

2: Exposure to endometrioma fluid

0.33 ± 0.19 c

0.18 ± 0.09 a

0.57 ± 0.21c

0.55 ± 0.32 c

3: Exposure to endometrioma fluid +Transferrin

0.42 ± 0.15 a,b

0.15 ± 0.05 a

0.35 ± 0.15 b

0.81 ± 0.47 a

4: Exposure to endometrioma fluid + Cysteamine and Cystine

0.37 ± 0.22 b,c

0.14 ± 0.03 a

0.38 ± 0.19 b

0.61 ± 0.43 c

  1. Within each column, data with different superscripts are different at p < 0.05. Each treatment was repeated four times with 5 oocytes for each time for detection of mitochondrial membrane polarization. To determine ADP/ATP levels within oocytes, 10 oocytes from each treatment were collected for each sample preparation