Transferrin and antioxidants partly prevented mouse oocyte oxidative damage induced by exposure of cumulus-oocyte complexes to endometrioma fluid

Table 3 Effects of endometrioma fluid, transferrin, cysteamine and cystine on GSH, ROS level and spindle morphology of mouse oocytes

Groups	GSH (pmol/oocyte)	ROS (Relative ratio)	Abnormal Spindles (%)
1:Control	3.43 ± 1.17 ^a	1.00 ± 0.33 ^a	17.2 ± 3.5 ^a
2: Exposure to endometrioma fluid	2.29 ± 1.01^b	1.63 ± 0.57 ^c	29.6 ± 5.4 ^c
3: Exposure to endometrioma fluid +Transferrin	2.38 ± 0.95 ^b	1.21 ± 0.53 ^b	23.8 ± 5.7 ^b
4: Exposure to endometrioma fluid + Cysteamine and Cystine	3.15 ± 1.36 ^a	1.15 ± 0.48 ^a,b	19.4 ± 4.5 ^a,b

Within each column, data with different superscripts are different at p < 0.05. Each treatment was repeated four times with 5 oocytes for each time for detection of ROS. To determine GSH content within oocytes, each treatment was repeated four times with 5 samples for each time, and 50 oocytes in one sample preparation. To determine the spindle morphology, each treatment was repeated four times with 30 oocytes for each time

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