Fig. 3

Associations among HOTAIRM1, miR-433-5p and PIK3CD were identified by biological experiments. a The binding sequences between HOTAIRM1 and miR-433-5p were exhibited. b The targeting sites between miR-433-5p and PIK3CD were presented. c Dual luciferase assay displayed that the luciferase activity was reduced or elevated in WT HOTAIRM1 group when miR-433-5p mimic or inhibitor treatment, **p < 0.01 vs. NC. d The activity in WT PIK3CD group was reduced or increased when treated by miR-433-5p mimic or inhibitor, **p < 0.01 vs. NC. e-g Overexpression of HOTAIRM1 enhanced the mRNA and protein expression levels of PIK3CD, while miR-433-5p mimic suppressed HOTAIRM1 expression. H-J. PIK3CD expression was decreased or increased with si-HOTAIRM1 or miR-433-5p inhibitor treatment, respectively. *p < 0.05, **p < 0.01 vs. control, #p < 0.05, ##p < 0.01 vs. HOTAIRM1 or si-HOTAIRM1, &p < 0.05, &&p < 0.01 vs. mimic or inhibitor