Fig. 3

Lnc-SNHG1 acted as a sponge for miR-216b-5p in A2780/Taxol cells. a Expression level of lnc-SNHG1 and miR-216b-5p in A2780/Taxol and its parental A2780 cells. b A2780/Taxol cells were transfected with SNHG1-specific siRNAs; SNHG1- siRNA-2 was used for further investigation. c, d qRT-PCR analysis showed that miR-216b-5p was negatively regulated by SNHG1 while SNHG1 was negatively regulated by miR-216b-5p. e-f Luciferase reporter assay indicated that miR-216b-5p reduced the luciferase activity of SNHG1-WT rather than SNHG1-mut. L A2780/Taxol cells were transfected with MS2-tagged SNHG1-WT and MS2-tagged SNHG1-mut, and then were assayed by RIP