Table 1 Sequences of primers used in qRT-PCR
Gene | Primer (5′ → 3′) | Annealing temperature (°C) | Efficiency |
|---|---|---|---|
GDF9 | F: TGGAGCATCCTTCAGCAC R: GCAGCCTCTTCTCCCACA | 57.2 | 98.8% |
BMP15 | F: TTTACCGCCATCATCTCCAA R: TTTCCAAGCGTTAGACATCA | 53.4 | 91.9% |
GAPDH | F: ACGGATTTGGTCGTATTGGG R: CGCTCCTGGAAGATGGTGAT | 57.4 | 101.6% |
β-actin | F: GACAGGATGCAGAAGGAGAT R: CTGCTTGCTGATCCACATCT | 53.5 | 99.6% |
HPRT | F: CCATTCCTATGACTGTAGAT R: CCAGTTAAAGTTGAGAGATCAT | 51.8 | 96.5% |