Fig. 2

Mediation effect of miR-140 on the viability and apoptosis of insulin-treated granulosa cells. After transfection with miR-140 inhibitor/mimic or NC-inhibitor/mimic, the cells were further treated with a high concentration of insulin (100 ng/ml). a qPCR analysis was carried out to detect the miR-140 level in granulosa cells that underwent different treatments. b The EdU assay showed miR-140 inhibitor or mimic affected the viability of insulin-treated granulosa cells. c, d MTT staining was used to determine the proliferation of the insulin-treated granulosa cell proliferation transfected with the miR-140 inhibitor, mimic or their controls. N = 3. *P < 0.05, **P < 0.01, ***P < 0.001 vs. indicated group