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Fig. 4 | Journal of Ovarian Research

Fig. 4

From: MiR-140 targets RAP2A to enable the proliferation of insulin-treated ovarian granulosa cells

Fig. 4

miR-140 targeting of the 3′-UTR of RAP2A. a Bioinformatic analysis revealed a miR-140 binding site in the RAP2A gene. b DLRA was performed following co-transfection of a luciferase reporter containing either WT (wild-type) or MU (mutant) RAP2A and a miR-140 mimic into HEK293T cells. Data were analyzed with ANOVA with Tukey post-hoc test. N = 3. *P < 0.05, **P < 0.01 vs. control group. c qPCR analysis validated RAP2A expression in human GC from PCOS patient (n = 3) and non-PCOS patients (n = 3). d qPCR and e WB revealed that the RAP2A levels in insulin-treated granulosa cells transfected with miR-140 inhibitor, mimic or their controls. N = 3. *P < 0.05, **P < 0.01 vs. indicated group

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