Fig. 3

a. DCN mRNA expression in MGCs cultures seeded in different concentration. MGCs collected during IVF procedure and cultured in different concentrations as indicated. After 48 h, cells were collected and Total mRNA was subjected to qPCR with DCN and b-actin (housekeeping gene) primers. Data represent the mean ± SEM of four independent experiments. Gene expression was calculated as fold change relative to 30,000 cell sample that was set to 1. Different letters represent a significant difference (P < 0.05).b The effect of DCN on KGN cell migration. Representative. Cells were cultured to full confluence and a scratch was inflicted with and without the addition of DCN. The cells were then cultured in starvation medium for additional 24 h. Images were taken at 0 h, 18 h, and 24 h. The results show representatives of time-lapse images of migrating KGN cells out of three independent experiments. Scale bar = 100 μM