Table 1 Sequences of the Primers Used in Real-Time PCR
From: Decreased ovarian function and autophagy gene methylation in aging rats
Gene | Forward primer | Reverse primer |
|---|---|---|
Atg5 | CACTGGGACTTCTGCTCCTG | TTCTTCAACCAAGCCAAACC |
Atg12 | AAACGAAGAAATGGGCTGTG | GAAGGGGCAAAGGACTGATT |
Atg16L | CTGTGCTTTTCCCGTCTTTC | GCCCTGATTTGGTTTCCAC |
Lc3B | GGTGTTTTTCTCCTGGTTTGG | GCACTTGGACTTCAGCCTTC |
Beclin1 | GCCTCTGAAACTGGACACG | CCTCTTCCTCCTGGCTCTCT |
Dnmt1 | CGACGACGCTAAGGACGATGATG | GCCTTGTTGCTCGCCTCTGTC |
Dnmt2 | GCGGTTGCGAGAGGATGGAAC | ACGTCAATAGCAGCCACCACATG |
Dnmt3B | AGACCAGAGGCCGCAGATCAAG | TCCGCTTCACCATCTCCATCTCC |
Dnmt3A | TGCCAGTCATCCGCCACCTC | CTCCGTCCTCTCGTTCTTGGTG |