Table 2 Sequences of the Primers Used in Methylation-Specific PCR
From: Decreased ovarian function and autophagy gene methylation in aging rats
Gene | Forward primer | Reverse primer |
|---|---|---|
Atg5-M | GTTGGTTTAAGAAGGAAATTAGCG | CCCGAACTACTCCTAAACGTA |
Atg5-U | GGTTTAAGAAGGAAATTAGTGGAT | ACAAAACCCAAACTACTCCTAAACATA |
Atg12-M | ATAGATTAGTTAGGGCGAGTAGCG | CCCGACTATTTCCTATCGAA |
Atg12-U | AGATTAGTTAGGGTGAGTAGTGAGT | TCAACCCAACTATTTCCTATCAA |
Atg16L-M | GTAAGGAAATAGATATTAGCGGAT | GACCGAAAACGTCGTAAAAC |
Atg16L-U | AAGTAGTAAGGAAATAGATATTAGTGGAT | AAACTCAACCAAAAACATCATAAAAC |
Lc3B-M | GGAGATATATAAGGGAAGTGATCGTC | GACGCTATTTAAAAATCTTCTCG |
Lc3B-U | AGGAGATATATAAGGGAAGTGATTGTT | CCAACACTATTTAAAAATCTTCTCA |
Beclin1-M | TTTATAAGAGAGTATGGACGGTTTC | CCGATCGACTAACTAAAAACTTC |
Beclin1-U | AATTTATAAGAGAGTATGGATGGTTTT | CCAATCAACTAACTAAAAACTTCC |