Fig. 2

Arginine depletion decreases ovarian cancer cell adhesion to collagen. a Representative micrographs of control, hArgI treated and hArgI + citrulline treated SKOV3 cells after fixation and staining with crystal violet to detect adhesion. Scale bar is 100 μm. Colorimetric quantification of the dissolved dye was performed at 560 nm using an Elisa plate reader. The bar graph represents the quantification of adherent cells expressed as fold increase relative to the untreated control. Significance was set at p < 0.05. b Representative micrographs of control, hArgI treated and hArgI + citrulline treated Caov-3 cells after fixation and staining with crystal violet to detect adhesion. Scale bar is 100 μm. Colorimetric quantification of the dissolved dye was performed at 560 nm using an Elisa plate reader. The bar graph represents the quantification of adherent cells expressed as fold increase relative to the untreated control. Significance was set at p < 0.05. c Dose adhesion response of SKOV3 cells to the indicated concentrations of hArgI. Data expressed as fold change relative to the untreated control. Significance was set at p < 0.05. d Control and hArgI treated cells were immunostained with vinculin (green) and Rhodamine phalloidin (orange). SKOV3 treated cells were imaged using a 63x objective of a fluorescent microscope. Scale bar = 10 μm. e Bar graphs illustrating the quantification of the area of focal adhesions (right panel) and the number of focal adhesions (left panel) were evidenced by vinculin staining and generated using the CLAHE and Log3D plugins in ImageJ. The area of focal adhesion is expressed in arbitrary unit (a.u.) and the number of focal adhesion is shown as absolute values of the means in every condition