Fig. 2
From: Lnc-GULP1–2:1 affects granulosa cell proliferation by regulating COL3A1 expression and localization
Effects of lnc-GULP1–2:1 on granulosa cell proliferation and related gene expression. Lv-lnc-GULP1–2:1 capable of overexpressing lnc-GULP1–2:1 (a). Cell proliferation was analyzed in group of Lv-EGFP and Lv-lnc-GULP1–2:1 at 0 h, 24 h, 48 h and 72 h by using CCK-8 (b). “0 h” means the beginning of cell attachment. The Ki-67 expression in KGN cell lines was analyzed by using immunofluorescence assay (c). Red signal, Ki-67. Blue signal, DAPI signal indicates nuclear localization. Bar = 50 μm. Real time PCR was used to detect the expression of genes related to cell proliferation, cell apoptosis and cell cycle (d). The GAPDH gene was used as an internal reference gene. Lv-EGFP, KGN control cell line overexpressing EGFP; Lv-lnc-GULP1–2:1, KGN cell line overexpressing lnc-GULP1–2:1. NS indicates no significant difference, * indicates p < 0.05, and*** indicates p < 0.0001