Skip to main content
Fig. 3 | Journal of Ovarian Research

Fig. 3

From: Lnc-GULP1–2:1 affects granulosa cell proliferation by regulating COL3A1 expression and localization

Fig. 3

Lnc-GULP1–2:1 overexpression affects the expression and localization of COL3A1 in granulosa cells. The expression correlation of lnc-GULP1–2:1 and COL3A1 was analyzed by real-time PCR in different cell lines (including Bewo, HTR-8/SVneo, OVCAR3, SKOV3, MDA-MB-231, Hep G2, 293 T, U87 MG and JAR) (a). The expression of COL3A1 from patient of normal, DOR and PCOS-derived granulosa cells (b & c). The effect of overexpression of lnc-GULP1–2:1 on COL3A1 mRNA and protein expression (d & e). The expression and localization of COL3A1 in KGN cell lines analyzed by immunofluorescence assay (f). Red signal, COL3A1 signal. Blue signal, DAPI signal indicates nuclear localization. Bar = 50 μm. The mRNA and protein expression of COL3A1 can be dose-dependently reduced by using COL3A1 specific siRNA (g & h). Knocking down of COL3A1 on the expression of lnc-GULP1–2:1 (i). GAPDH was used as an internal control. NS indicates no significant difference, * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.0001

Back to article page