Fig. 4
From: Lnc-GULP1–2:1 affects granulosa cell proliferation by regulating COL3A1 expression and localization
Effect of COL3A1 expression on granulosa cell proliferation and related gene expression. KGN cell proliferation was inhibited by knocking down of COL3A1 expression in dose-dependent manner (a). Effects of simultaneous silencing of COL3A1 expression on cell proliferation in Lv-lnc-GULP1–2:1 and Lv-EGFP cells (b). The expression of Bcl-2, Bcl-XL, Bax, CCND2 and p16 in Lv-lnc-GULP1–2:1 and Lv-EGFP cells by knockdown of COL3A1 (c). GAPDH was used as an internal control. The concentration of siRNA in plots b and c is 20 nM. NC, negative control. si-NC, silence control. NS indicates no significant difference, * indicates p < 0.05, ** indicates p < 0.01, and *** indicates p < 0.0001. Primary granulosa cells were infected with 5 × 1010 PFU/ml adenovirus overexpress lnc-GULP1–2:1 (adv-lnc-GULP1–2:1) or its control (adv-control), and flow cytometry was used to detect cell cycle changes on cells treated with adenovirus after 48 h (d)