Fig. 1
Identification of exosomes from human ovarian cancer cell line HO8910 and HO8910PM. a and b migration and invasion assay. 2 × 105 and 3 × 105 of HO8910 and HO8910PM cells were respectively added to trans-well plates without or with Matrigel-coated, 24 h later, migration and invasion were assessed by trans-well assays. Results in A were representative of three experiments with similar results. Results in B were shown as means ± SEM for three separate experiments. *** p < 0.001. c Cell proliferation assay. 4 × 103 of HO8910 and HO8910PM cells were plated onto 96-well plates. Cell proliferation in 24 h, 48 h,72 h,96 h was respectively assessed by CCK8 assays. Results were shown as means ± SEM for three separate experiments. d Transmission electron microscopy. Transmission electron micrographs of purified exosomes secreted from HO8910 and HO8910PM cell lines. Scale bar, 200 nm and 100 nm. e Nanoparticle analysis. Concentration and size distribution of nano-sized particles in exosome suspension were measured using Litesizer 500. f Western blot analysis. Exosomes, isolated from HO8910 and HO8910PM cell lines, and parental cellular lysates (10 μg/lane) were western blotted for HSP70, CD63, CD9 and CD81.Actin served as the control