Fig. 2
Exosomes derived from HO8910PM cells enhance aggressive phenotypes of HO8910 cells. a-b Confocal microscopy. 5 × 104 HO8910 cells were cultured with exosomes labeled green fluorescent dye PKH67 for 4 h or 24 h. HO8910 cells were observed under confocal microscopy at 600 × magnification. DAPI was used to stain the nuclei and Palloidin was used to stain the cell cortex. Scale bar 20 μm. b Area fraction (%Area) reflected the degree of PKH67 labeled exosomes uptaking by recipient cells. Data represented the means ± SEM of three independent experiments. ****p < 0.0001, *p < 0.05 compared to 0 h. c-d Migration and invasion assays.10μg/mL of exosomes extracted from HO8910PM and HO8910 cells were incubated with HO8910 cells in 6-well plates for 48 h. PBS served as the control. 2 × 105 and 3 × 105 digested HO8910 cells were respectively added to trans-well plates without or with Matrigel-coated. Migration and invasion were assessed by trans-well assays after 24 h. Results in B were representative of three experiments. Results in D were shown as means ± SEM for three separate experiments. One-way ANOVA was used to analyze the differences. **p < 0.01, *p < 0.05