Fig. 3

Identification of molecules in donor exosomes affecting phenotypes of recipient cells. a Western blot analysis on expression levels of CD44 in HOExos and PMExos (10 μg/lane). Both CD63 and Actin were used as internal controls. The blots shown were representative of three separate experiments. b Western blot analysis on expression levels of CD44 in HO8910 and HO8910PM cells (10 μg/lane). The blots shown were representative of three separate experiments. c 10μg/ml of exosomes extracted from HO8910PM and HO8910 cells were incubated with HO8910 cells in 6-well plates for 48 h. Adding PBS served as control. Thereafter, HO8910 cell lysates were collected, lysed and immunoblotting were performed (10 μg/lane). Actin was used as the internal control. The blots shown were representative of three separate experiments. d Real-time qRT-PCR. 10μg/ml of exosomes extracted from HO8910PM and HO8910 cells were incubated with HO8910 cells in 6-well plates for 48 h. PBS served as the control. Extracted RNA of HO8910 cells was used for qRT-PCR analysis. CD44 mRNA was detected by RT-PCR. GAPDH was used as the internal control. Results were shown as means ± SEM for three separate experiments. NS meant no significant difference