Fig. 2

Silencing of UCA1 attenuated the pathological characteristics of PCOS mice. Lentivirus containing sh-UCA1 or sh-NC was infected into the ovary of the PCOS mice, control mice infected with lentivirus carrying an empty negative control vector. a H&E staining result of the ovary. Scale bar: 100 μm. b Number of granular cells per follicle. c The serum testosterone level was downregulated by UCA1 silencing. d The expression level of UCA1 in normal (Con) and PCOS mice. LncRNA UCA1 expression was enhanced in PCOS mice and decreased after sh-UCA1 treatment. e The release level of serum insulin was increased in PCOS mice and sh-UCA1 downregulated the release. f Area under the curve (IAHC) of serum insulin level. Data were mean ± SD (n = 6), #p < 0.05 showed significant difference vs. control, *p < 0.05 showed significant difference vs. PCOS-sh-NC.