Fig. 2

LCK inhibitors chemosensitize cisplatin resistant endometrioid cells and increase apoptosis. Cisplatin resistant ovarian endometrioid cells (CP70) were cultured and pretreated with an LCK inhibitor (saracatinib) or vehicle, followed by vehicle or combination LCKi-cisplatin, followed by cell viability assay performed with the CellTiterGlo Assay (a). Caspase 3/7 Assay was then performed to assess apoptosis (b). A second cisplatin resistant endometrioid cell line (HEC1a) was similarly treated and tested with subsequent proliferation and apoptosis assays performed (c, d). Cisplatin sensitive ovarian endometrioid cells (A2780) were cultured and treated according to the aforementioned paradigm (e). An alternative LCK inhibitor (PP2) was utilized for pretreatment in CP70 cells followed by co-treatment with PP2-cisplatin (f). All data represent at minimum three independent experiments with three technical replicates