Fig. 3

Cisplatin resistant endometrioid cells treated with LCK inhibitors indicate decreased P-LCK and ovarian endometrioid cells treated with LCK inhibitor indicate increased DNA double strand breaks. Cisplatin resistant ovarian endometrioid cancer cells (CP70) were treated with DMSO, LCK inhibitor saracatinib (Sar) or PP2 at indicated doses for 48 h. Protein lysates were then immunoblotted for phosphorylated LCK (P-LCK Y394) and total LCK (T-LCK). Fold changes of protein expression are shown in the figure, with values normalized to vehicle control. GAPDH was used as loading control (a). CP70 cells treated with the indicated varied doses of saracatinib (Sar) were immunoblotted for 훾H2AX. Fold changes of protein expression are shown in the figure, with values normalized to vehicle control. GAPDH was used as a loading control (b). Each experiment was performed with at least three technical replicates