Fig. 1

Isolation of nuclear and cytoplasmic fractions. a Isolated nuclei were visualized at 400X magnification to confirm purity, left-to-right: phase contrast, Hoescht 33342, and trypan blue. b Western blot analysis for whole cell, cytoplasmic (cyt) and nuclear (nuc) extracts from pig granulosa cells using anti-Lamin B and anti-GAPDH antibodies. Lamin B and GAPDH were used as a nuclear or cytoplasmic marker protein, respectively. 50 μg of whole lysate and cytoplasmic extract or 100 μg of nuclear extract was loaded. c RT-qPCR of nuclear and cytoplasmic RNA fractions was performed on the small nuclear RNA U6. Data represents the mean ± SEM from three independent experiments