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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Short‐term rapamycin administration elevated testosterone levels and exacerbated reproductive disorder in dehydroepiandrosterone‐induced polycystic ovary syndrome mice

Fig. 1

Phenotypic evaluation of control; dehydroepiandrosterone (DHEA), and DHEA + rapamycin mice. a Representative estrous cyclicity assessment in 5-week-old female mice for eight consecutive days by vaginal cytology. The graph shows representative cycles from the three groups. Each box indicates one animal, and dots represent a day. M, metestrus; D, diestrus; P, proestrus; E, estrus. b Data are presented in a violin plot showing the frequency distribution curves. c Ovarian morphology by H&E staining. d Mean ovarian weights. Analysis of serum concentrations of testosterone (e), luteinizing hormone (LH) (f), follicle-stimulating hormone (FSH) (g), and LH/FSH (h) as measured by ELISA (n = 7–8/group). The data are presented as means ± SEM

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