Fig. 1

Inhibition of SphK1 suppressed adipocyte-induced EOC cell proliferation. SKOV3 (a) and A2780 (b) cells were serum starved overnight and cultured with serum-free medium (SFM) or adipocyte-conditioned medium (Adi-CM) in the presence or absence of PF543 (10 μM) for 48 h. Cell proliferation was measured using CCK-8 assay. c SphK1 mRNA was measured by quantitative RT-PCR 24 h after siRNA transfection. d SphK1 protein levels were determined by western blot analysis 48 h after siRNA transfection. SKOV3 (e) and A2780 (f) cells were transfected with the indicated siRNAs, followed by culture with SFM or Adi-CM for 48 h. Cell proliferation was measured by CCK-8 assay. The data are presented as the means±SD. *, P < 0.05 vs control; #, P < 0.05 vs Adi-CM alone