Fig. 2
From: Activation of SphK1 by adipocytes mediates epithelial ovarian cancer cell proliferation
Adipocytes activated SphK1/ERK signal in EOC cells. a Serum-starved SKOV3 and A2780 cells were cultured with SFM or Adi-CM for 24 h. SphK1 phosphorylation was determined by western blot analysis. Densitometric analysis of pSphK1 (normalized to total SphK1) is shown on the right. b SKOV3 cells were transfected with the indicated siRNAs and cultured with SFM or Adi-CM for 24 h. Levels of total and phosphorylated ERK (pERK) were determined by western blot analysis. The right panel shows densitometric analysis of pERK (normalized to total ERK). The data are presented as the means±SD. *, P < 0.05