Fig. 3From: Activation of SphK1 by adipocytes mediates epithelial ovarian cancer cell proliferationAdipocyte-induced SphK1 activation was ERK dependent. SKOV3 (a) and A2780 (b) cells were serum starved overnight, pretreated with U0126 (5 μM) for 2 h and then cultured with SFM or Adi-CM for 24 h. Levels of total and phosphorylated SphK1 (pSphK1) were determined by western blot analysis. Right panels show densitometric analysis of pSphK1 (normalized to total SphK1) corresponding to the bands shown in the western blots. The data are presented as the means±SD. *, P < 0.05Back to article page