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Fig. 1 | Journal of Ovarian Research

Fig. 1

From: Evaluation of mitochondria in mouse oocytes following cisplatin exposure

Fig. 1

The distribution of mitochondria in small and growing immature oocytes from saline and cisplatin (2 and 4 mg/kg) treated mice. Immature oocytes were isolated from saline treated control mice, or 3, 6 and 24 h after cisplatin and mitochondrial distribution was assessed using MitoTracker Green (green) (N = 3–4 mice per group). Mitochondrial localisation in small oocytes treated with saline or cisplatin 2 mg/kg (A) or 4 mg/kg (B), and growing oocytes treated with saline or cisplatin 2 mg/kg (C) or 4 mg/kg (D). Scale bar = 20 μm. E Magnification of small (a-b, scale bar = 10 μm) and growing oocytes (c-d, scale bar = 20 μm). F Percentage of growing oocytes with aggregated mitochondria for controls (n = 88) and 3 (n = 28), 6 (n = 32), 24 (n = 31) hours after 2 mg/kg cisplatin and G controls (n = 144) and 3 (n = 45), 6 (n = 43), 24 (n = 60) hours after 4 mg/kg cisplatin. No significant differences were observed (Kruskal-Wallis test, p-value > 0.05). Relative MitoTracker intensity of small oocytes treated with saline or cisplatin 2 mg/kg (H) or 4 mg/kg (I), and growing oocytes treated with saline or cisplatin 2 mg/kg (J) or 4 mg/kg (K). For 2 mg/kg cisplatin, small oocyte number n = 46/48 at 3 h, n = 35/29 at 6 h and n = 41/44 at 24 h after cisplatin treatment; growing oocyte number n = 35/35 at 3 h, n = 31/33 at 6 h and n = 37/39 at 24 h after cisplatin treatment. For 4 mg/kg cisplatin, small oocyte number n = 45/50 at 3 h, n = 72/75 at 6 h and n = 35/22 at 24 h after cisplatin treatment; growing oocyte number n = 38/41 at 3 h, n = 37/41 at 6 h and n = 51/45 at 24 h after cisplatin. t-test for comparison of treated oocytes with relevant controls at each time point

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